A new High Sensitive Functional Nephelometrical Assay for Assaying C- reactive protein in Serum Based on Phosphocholine Interaction
نویسندگان
چکیده
C-reactive protein (CRP) is able to bind phosphocholine in the presence of calcium ions. According to a previous functional property of CRP, we tried to develop an affordable and cheap high sensitive nephelometric CRP assay using soy oil. Serum samples were measured by Nephelometer BNII (Siemens), by mixing the serum with diluted soy oil emulsion (Intralipid ® 20%) and Tris-calcium buffer (PH 7.5). The measurement took place after 12 min incubation time at 37°C by measuring the agglutination between CRP and phosphocholine. Results from our automated functional assay were compared with results obtained using an immunoturbidimetric CRP assay. Results showed a good correlation coefficient for method comparison between functional nephelometric CRP assay and immunoturbidimetric CRP assay, r = 0.895, significance level p <0.0001. The limit of detection for the functional nephelometric CRP assay was 0.1 mg/L. However, the within run % CV values for the functional assay were 6.1 % (20 mg/L), 4.7 % The new functional nephelometric CRP assay enables high sensitive CRP measurement in serum in the range of 0.1 mg/L to 300 mg/L. The functional assay could be used for veterinary analysis due to the ability to measure CRP according to the functional properties, not the morphological properties which depend on specific antibodies.
منابع مشابه
A new high-sensitive nephelometric method for assaying serum C-reactive protein based on phosphocholine interaction.
BACKGROUND The measurement of C-reactive protein (CRP) concentrations has been of interest as a classical marker of acute phase response; in addition, it has been of particular interest in cardiovascular risk stratification where high-sensitive measurements are necessary. Since CRP is able to bind phospholipids (mainly phosphocholine) in the presence of calcium ions, we explored the possibiliti...
متن کاملA new turbidimetric method for assaying serum C-reactive protein based on phosphocholine interaction.
BACKGROUND C-reactive protein (CRP) is able to bind phospholipids (mainly phosphocholine) in the presence of calcium ions. We investigated the use of this property for developing an affordable turbidimetrical CRP assay based on diluted soy oil. METHODS Serum (or heparinized plasma) was mixed with Intralipid 20% in Tris-calcium buffer (pH 7.5). After 30 min of incubation at 37 degrees C, the C...
متن کاملSerum Level of High Sensitive C-Reactive Protein in Normal and Preeclamptic Pregnancies
Background and Objective: The aim of this study was to determine the level of plasma high sensitive c-reactive protein (hs-CRP) in preeclampsia and to compare hs-CRP levels between normal pregnant women, mild preeclamptic, and severe preeclamptic women. Materials and Methods: Serum hs-CRP levels were inv...
متن کاملMid-Trimester Amniotic Fluid High Sensitive C-Reactive Protein Level in the Prediction of Preterm Delivery
Background and Objectives: Preterm labor is a serious problem in obstetrics, accounting for 70% of perinatal mortality. High sensitive C - reactive protein (HS-CRP) is a sensitive marker of Inflammation. Our aim in this study was to determine Amniotic fluid hs-CRP concentration and its correlation with pre-Term delivery. Materials and methods: This prospective study was conducted on 90 p...
متن کامل